Year: 2017 | Month: December | Volume 7 | Issue 6
Identification of Biofilm Forming Salmonella Species of Layers from Tribal Area of Hoshangabad District of Madhya Pradesh
Abstract:
The present experiment was designed to identify the biofilm forming Salmonella organism from cloacal swab, dry faeces, feed and water of layers in tribal area of Hoshangabad district, in rainy, winter and summer seasons. A total of 181 samples consisting of cloacal swab (109), dry faeces (18), feed (18), water (18) and egg shell (18) were collected in buffered peptone water in all the three seasons. For isolation, selective enrichment was performed in rappaport vassiliadis broth followed by streaking in macConkey, brilliant green agar, xylose lysine deoxycholate media. Phenotypically they were identified by motility followed by Grams staining and biochemical tests like oxidase, catalase, indole, methyl red, Voges Proskauer, triple sugar iron agar and urease. Further they were confirmed by latex agglutination test. Phenotypically identified 17 presumptive Salmonella isolates were subjected to genotypic characterization using 16S rRNA, invA gene, Salmonella enterica serovar Typhimurium gene and Salmonella enterica serovar Gallinarum-Pullorum by PCR. All the isolates were positive (100%) for 16S rRNA and invA gene, Salmonella enterica serovar Typhimurium gene were found to be 64.70%, while 35.29% isolates remain unidentified. Phenotypically and genotipically confirmed Salmonella isolates were subjected to biofilm formation. The percentagewise isolation of Salmonella organism from cloacal swabs were 14.63%, 5.26% and 10.00% in rainy, winter and summer season, respectively. While in rainy season from dry faeces, water samples and egg samples were 33.33%. The overall incidence of salmonellosis in layers from tribal area of Hoshangabad district was 9.39% (17/181) and all of them were biofilm producers.
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